Bicarbonate
Bicarbonate
Categories
- Chemistry
- Health Assessment and Safety
General Information
| Name | Information |
|---|---|
| Code: | T530 |
| Matrix: | Serum / Plasma |
| Application: | Insurance Risk Assessment, Wellness |
| Turnaround Time: | Daily |
| Analytical - Method: | Bicarbonate reacts with phosphoenolpyruvate (PEP) in the presence of PEPC to produce oxaloacetate and phosphate: PEPC PEP + HCO3- oxaloacetate + H2PO4- The above reaction is coupled with one involving the transfer of a hydrogen ion from NADH analog to oxaloacetate using MDH. MDH Oxaloacetate + NADH analog + H+ malate + NAD+ analog The resultant consumption of NADH analog causes a decrease in absorbance, which is proportional to the concentration of bicarbonate in the sample being assayed. |
| Reportable - Test Results Type: | Quantitative |
| Units - Conventional: | mmol/L |
Specimen Information
| Name | Information |
|---|---|
| Minimum Volume: | 300 µL |
| Preferred Volume: | 500 µL |
| Rejection Criteria: | Improper labeling, improper sample handling, and wrong sample type. |
| Collection Instructions: | See “Blood Collection” |
| Collection Patient Preparation: | NA |
| Container: | Only the specimens listed below were tested and found acceptable. Serum Plasma: Li‑heparin plasma |