Bicarbonate
Bicarbonate
Categories
- Chemistry
- Health Assessment and Safety
General Information
Name | Information |
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Code: | T530 |
Matrix: | Serum / Plasma |
Application: | Insurance Risk Assessment, Wellness |
Turnaround Time: | Daily |
Analytical - Method: | Bicarbonate reacts with phosphoenolpyruvate (PEP) in the presence of PEPC to produce oxaloacetate and phosphate: PEPC PEP + HCO3- oxaloacetate + H2PO4- The above reaction is coupled with one involving the transfer of a hydrogen ion from NADH analog to oxaloacetate using MDH. MDH Oxaloacetate + NADH analog + H+ malate + NAD+ analog The resultant consumption of NADH analog causes a decrease in absorbance, which is proportional to the concentration of bicarbonate in the sample being assayed. |
Reportable - Test Results Type: | Quantitative |
Units - Conventional: | mmol/L |
Specimen Information
Name | Information |
---|---|
Minimum Volume: | 300 µL |
Preferred Volume: | 500 µL |
Rejection Criteria: | Improper labeling, improper sample handling, and wrong sample type. |
Collection Instructions: | See “Blood Collection” |
Collection Patient Preparation: | NA |
Container: | Only the specimens listed below were tested and found acceptable. Serum Plasma: Li‑heparin plasma |