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Bicarbonate

Categories

  • Chemistry
  • Health Assessment and Safety

General Information

Code:T530
Matrix:Serum / Plasma
Application:Insurance Risk Assessment, Wellness, Clinical Trials
Turnaround Time:Daily
Analytical - Method:Bicarbonate reacts with phosphoenolpyruvate (PEP) in the presence of PEPC to produce oxaloacetate and phosphate: PEPC PEP + HCO3- oxaloacetate + H2PO4- The above reaction is coupled with one involving the transfer of a hydrogen ion from NADH analog to oxaloacetate using MDH. MDH Oxaloacetate + NADH analog + H+ malate + NAD+ analog The resultant consumption of NADH analog causes a decrease in absorbance, which is proportional to the concentration of bicarbonate in the sample being assayed.
Reportable - Test Results Type:Quantitative

Shipping Information

Units - Conventional:mmol/L

Specimen Information

Minimum Volume:300 µL
Preferred Volume:500 µL
Rejection Criteria:

Improper labeling, improper sample handling, and wrong sample type.

Collection Instructions:

See “Blood Collection”

Collection Patient Preparation:NA
Container:Only the specimens listed below were tested and found acceptable. Serum Plasma: Li‑heparin plasma