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Blood Urea Nitrogen

Categories

  • Chemistry
  • Health Assessment and Safety

General Information

Code:T502
A.K.A.:Urea
Matrix:Serum / Plasma
Application:Insurance Risk Assessment, Wellness, Clinical Trials
Turnaround Time:Daily
Analytical - Method:Kinetic test with urease and glutamate dehydrogenase. Urea is hydrolyzed by urease to form ammonium and carbonate. Urease Urea + 2 H2O 2 NH4+ + CO32- In the second reaction 2-oxoglutarate reacts with ammonium in the presence of glutamate dehydrogenase (GLDH) and the coenzyme NADH to produce L-glutamate. In this reaction two moles of NADH are oxidized to NAD+ for each mole of urea hydrolyzed. GLDH NH4+ + 2-oxoglutarate + NADH L-glutamate + NAD+ + H2O The rate of decrease in the NADH concentration is directly proportional to the urea concentration in the specimen and is measured photometrically.

Shipping Information

Units - Conventional:mg/dL

Specimen Information

Minimum Volume:300 µL
Preferred Volume:500 µL
Rejection Criteria:

Improper labeling, improper sample handling, and wrong sample type.

Collection Instructions:

See “Blood Collection”

Collection Patient Preparation:NA
Container:Only the specimens listed below were tested and found acceptable. Serum Plasma: Li-heparin and K2-EDTA plasma. Do not use ammonium heparin.