Blood Urea Nitrogen
Blood Urea Nitrogen
Categories
- Chemistry
- Health Assessment and Safety
General Information
| Name | Information |
|---|---|
| Code: | T502 |
| A.K.A.: | Urea |
| Matrix: | Serum / Plasma |
| Application: | Insurance Risk Assessment, Wellness |
| Turnaround Time: | Daily |
| Analytical - Method: | Kinetic test with urease and glutamate dehydrogenase. Urea is hydrolyzed by urease to form ammonium and carbonate. Urease Urea + 2 H2O 2 NH4+ + CO32- In the second reaction 2-oxoglutarate reacts with ammonium in the presence of glutamate dehydrogenase (GLDH) and the coenzyme NADH to produce L-glutamate. In this reaction two moles of NADH are oxidized to NAD+ for each mole of urea hydrolyzed. GLDH NH4+ + 2-oxoglutarate + NADH L-glutamate + NAD+ + H2O The rate of decrease in the NADH concentration is directly proportional to the urea concentration in the specimen and is measured photometrically. |
| Units - Conventional: | mg/dL |
Specimen Information
| Name | Information |
|---|---|
| Minimum Volume: | 300 µL |
| Preferred Volume: | 500 µL |
| Rejection Criteria: | Improper labeling, improper sample handling, and wrong sample type. |
| Collection Instructions: | See “Blood Collection” |
| Collection Patient Preparation: | NA |
| Container: | Only the specimens listed below were tested and found acceptable. Serum Plasma: Li-heparin and K2-EDTA plasma. Do not use ammonium heparin. |