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Cocaine metabolite, Saliva

Categories

  • Drugs of Abuse
  • Risk Assessment
  • Toxicology

General Information

Code:T536
Matrix:Saliva
Application:Insurance Risk Assessment
Turnaround Time:Daily
Analytical - Method:competitive micro-plate immunoassay
Reportable - Test Results Type:Qualitative

Shipping Information

Specimen Information

Minimum Volume:The minimum saliva volume necessary for Cocaine testing is 50 uL. However, there is a priority of testing when small volumes are obtained with 1) HIV and 2) Amylase being first, then 3) Cocaine. So it would actually take at least 140 uL of sample to perform these three tests. If there is < 140 uL, then the Cocaine would have to be QNS’d.
Rejection Criteria:

Grossly discolored samples or specimens that have gross particulate matter should be reported out as NSA in LIS with a message code M067. Colorless specimens should be reported out as NSA in LIS with a message code M077.

Collection Instructions:

Saliva from an OraSure® HIV-1 Oral Specimen Collection Device is the recommended specimen. The consent, collection device, and a coordinating clean polypropylene 16 x 75 tube is barcoded by ILS setups. ILS Setups then prepares the sample for testing as follows. The collection device is held with the tip in the upright position, and then the tip is broken off. This end is then mounted onto the empty polypropylene tube. The original swab collection device and empty tube are then spun in the centrifuge at 2000 rpms for 5 minutes. The actual saliva testing specimen transfers to the polypropylene tube during the centrifugation process. The tubes are pulled apart from each other. The actual testing saliva specimens are racked into Hamilton primary racks for initial testing while the corresponding swab is racked in cardboard boxes mirroring the Hamilton rack.

Collection Patient Preparation:NA
Container:Saliva is a complex mixture of parotid, submandibular, sublingual and minor salivary gland secretions mixed with mucin, bacteria, leukocytes, sloughed epithelial cells and gingival crevicular fluid.(5) The fact that cocaine and cocaine metabolites are present in oral fluid following human use has been documented.(3) The InterceptÒ Oral Specimen Collection Device was developed for the purpose of collecting oral fluid for diagnostic testing. The collection device consists of a treated absorbent cotton fiber pad affixed to a nylon stick (Collection Pad) and a preservative solution in a plastic container (Specimen Vial). The Collection Pad is impregnated with a mixture of salts and gelatin which creates a hypertonic environment and an increase osmotic pressure wherever it contact oral mucosal cells. The pad is placed in contact with the gingival mucosa (between the lower gum and cheek) which enhances the flow of mucosal transudate across the mucosal surfaces onto the absorptive cotton fibers of the pad. Following the collection period, the Collection Pad is placed into a vial containing a preservative solution which serves to inhibit the growth of oral microorganisms recovered on the Collection Pad. The vial is sealed with a plastic cap and transported to a laboratory for processing and testing. Following processing, a fluid containing a mixture of mucosal transudate, saliva components and the preservative solution is recovered which is suitable for testing for the presence of cocaine an cocaine metabolites in the OTI Cocaine Metabolite InterceptÒ MICRO-PLATE EIA. Refer to the InterceptÒ Oral Specimen Collection Device product insert for specific instructions on the proper collection, handling, and adequacy of oral fluid samples.