- Drugs of Abuse
- Risk Assessment
|Application:||Insurance Risk Assessment, Wellness, Clinical Trials|
|Analytical - Method:||enzyme-linked immunosorbent assay (ELISA)|
|Reportable - Test Results Type:||Qualitative|
|Minimum Volume:||The volume of oral fluid necessary for HIV-1 testing is 75 uL. However, there generally is a panel of four tests performed on oral fluid and there is a specific priority of testing when small volumes are encountered. Amylase and HIV have the first and second, then cocaine is third, and cotinine is fourth. A minimum of 190 µL of sample is required to perform all four tests.|
Grossly discolored samples or specimens that have gross particulate matter should have all tests cancelled with the comment NSA in Labware. Oral Fluid samples that do not contain adequate volume should have their tests cancelled in Labware as QNS.
1. Completely open the outer package of the OraSure HIV-1 oral specimen collection device. 2. Peel open pad package far enough to allow easy removal of the collection pad. 3. Instruct the patient to remove the pad from the pouch by grasping the nylon stick and gently pull it out of the package. 4. Instruct the patient to place the pad in the mouth between the lower cheek and gum and gently rub back and forth until moist. 5. Instruct the patient to keep the pad in place for two minutes (maximum 5 minutes) while timing. 6. While timing the two minutes, remove the specimen vial from the outer package. Holding the vial upright with the pointed tip down, carefully remove the cap from the vial using a bending or rocking motion to prevent spilling. 7. After two minutes, instruct patient to remove the pad from the mouth, insert the pad into the vial, pushing it all the way to the bottom of the vial. 8. Instruct patient to break the scored pad handle by snapping it against the side of the vial. 9. Replace the cap. Make sure you hear the “snap” sound—this means the cap is properly seated in the vial. Affix test number sticker to the specimen vial. It is critical that the identifier number on the test requisition and on the specimen vial are exactly the same. NOTE: The OraSure collection pad may taste salty and some patients may experience a dry sensation in the mouth.
|Collection Patient Preparation:||NA|
|Container:||OraSure® HIV-1 Oral Specimen Collection Device|