- Health Assessment and Safety
- Urine Microscopy
|Insurance Risk Assessment, Wellness
|Analytical - Method:
|The iQ200 aspirates samples, collects images from samples using digital image capture of particles presented in a flow microscope, and performs image processing to isolate individual particles.
|Reportable - Test Results Type:
|The iQ200 is designed to provide uniform specimen flow past the microscope objective. The true volume is determined by using a solution having a known concentration of particles and counting the number of those particles seen per frame. Thus, the known particle concentration divided by the particle count per frame equals the volume per frame. Since the volume per frame is consistent and always known, particle counts are accurately measured as particles per microliter by the system, and can be reported in this manner, if desired. Results for microscopic particles can also be reported as particles per field of view, as typically done in urinalysis, if the equivalent volume observed in one field with slide microscopy is known. The volume of one field of view in manual microscopy was determined experimentally by measuring the average volume of a drop of normal urine delivered by a Pasteur pipette. This volume spreads out beneath the cover slip. This volume divided by the area of the cover slip determines the thickness through which particles settle before they are viewed on the slide. The numerical aperture of the objective lens determines the area viewed at any one time. This area multiplied by the average wet mount thickness, is the effective observed volume equivalent seen in one field of view.
|The minimum volume of liquid specimen for analysis by both the AX4280 and the iQ200 is 4.0mL. The iQ200 alone requires 3.0mL, the AX-4280 alone requires 2.0mL
|The specimen volume should not exceed 6.0mL.
Hematuria • Gross hematuria may cause incorrect results in subsequent samples. Do not test specimens exhibiting gross hematuria. This specimen type can’t be tested • Specimens with Gross Hematuria should be NSA’d
Random samples are collected in a suitable plastic or glass tube. Samples are shipped preserved at ambient temperatures and analyzed within 24 hours of receipt. If samples are not analyzed within 24 hours of receipt, they are stored at 2-8oC until analysis can be performed. To preserve urine elements and inhibit bacterial growth, a urine preservative tablet is added to each urine specimen upon collection. Stabilur urine preservative tablets (Cargille Laboratories) contain: Monopotassium phosphate Benzoic acid Sodium benzoate Hexamethylenetetramine Mercuric oxide (0.36%) Sodium Bicarbonate (3.62%) Stabilur urine preservative tablets have not been found to cause interference with testing. Bring the specimen to room temperature before testing. Mix specimen well before testing. Do NOT add disinfectant or detergent to the specimen. Keep specimens out of direct sunlight. Do NOT centrifuge urine specimens.
|Collection Patient Preparation:
|Freshly voided urine samples should be collected in plastic container